Peer-reviewed veterinary case report
Validation of a commercial real-time PCR kit for specific and sensitive detection of Pseudorabies.
- Journal:
- Journal of virological methods
- Year:
- 2013
- Authors:
- Pol, Françoise et al.
- Affiliation:
- Anses · France
Plain-English summary
This study looked at a new test kit called the ADIAVET PRV REALTIME kit, which is designed to detect the Pseudorabies virus, a virus that primarily affects pigs but can also impact other animals. The kit can analyze samples from pigs, like nasal swabs or tissue from the tonsils, brain, or lungs, as well as samples from other animals, including pets like dogs and cats. Researchers tested how well the kit works compared to the traditional method of isolating the virus, which is considered the best way to diagnose the infection. The results showed that the kit is both specific and sensitive, meaning it can accurately detect the virus without false results. Overall, the ADIAVET PRV REALTIME kit was fully validated for use in diagnosing Pseudorabies.
Abstract
Pseudorabies virus is the causative agent of Aujeszky's disease, one of the OIE listed diseases that mainly affects swine, but also can affect other animal species, and which can lead to heavy economic losses in pig industry. This study was designed to evaluate the performance of the ADIAVET(®) PRV REALTIME kit, a new commercial real time PCR kit for Pseudorabies virus genome detection developed by the French manufacturer Adiagène. It can be used on pig biological samples such as nasal swab supernatant, tonsil, brain or lung samples, or on samples from other susceptible animals, such as domestic carnivores. This ready-to-use duplex PCR assay contains an external positive control, appropriate for assessing DNA extraction efficiency and the presence of PCR inhibitors. The analytical specificity and sensitivity, intra- and inter-assay repeatability and diagnostic characteristics of the kit were determined and compared with virus isolation, which is the gold standard. Based on these results, the ADIAVET(®) PRV REALTIME kit received full validation for diagnostic purposes.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/23174164/