A clonality analysis based on T-cell receptor beta and delta loci for high-grade gastrointestinal lymphoma in dogs.

Abstract

Clonality assays based on antigen receptors are used as adjunct examinations in the diagnosis of lymphoproliferative diseases. We investigated the usefulness of the T-cell receptor beta () and T-cell receptor delta () loci in clonality assays for high-grade gastrointestinal (GI) lymphoma in dogs. For, we used primers reported previously; for, we designed primers for each of theandgenes based on genomic sequences. Genomic DNA was extracted from 39 formalin-fixed, paraffin-embedded sections of high-grade GI lymphoma diagnosed histologically. The sensitivity ofandprimers for GI lymphoma was 41.0% and 38.5%, respectively, which was lower than the 82.1% sensitivity of T-cell receptor gamma () primers However, some cases that could not be detected usingprimers had clonality with eitherorprimers. We found thelocus to be more suitable as a first choice for the assay of canine lymphoma clonality than theandloci. However, the detection rate of T-cell clonality may be enhanced usingandprimers for lymphoma cases not detected usingprimers.