Peer-reviewed veterinary case report
Minimally invasive spleen biopsy method for dogs with Leishmania
By Santos, Silvana Ornelas et al.·Published in Comparative immunology, microbiology and infectious diseases·2016·Hospital de Medicina Veteriná, Brazil·View original on PubMed →
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Original publication title: A minimally invasive approach to spleen histopathology in dogs: A new method for follow-up studies of spleen changes in the course of Leishmania infantum infection.
- Species:
- dog
Plain-English summary
A group of dogs with Leishmania infection showed changes in their spleen tissue compared to uninfected dogs. The infected dogs had fewer lymphoid follicles and more plasma cells, which are indicators of immune response, along with signs of inflammation like granulomas. Researchers developed a new, less invasive method to collect spleen tissue samples, making it easier to study these changes without needing surgery. This technique could help veterinarians monitor dogs with Leishmania and other diseases more effectively.
People also search for: dog spleen problems · Leishmania infection in dogs · dog spleen biopsy method
Abstract
Severe forms of zoonotic visceral leishmaniosis (ZVL) are associated with disruption of the spleen structure. However, the study of spleen histology requires splenectomy or necropsy. In this work, we present a minimally invasive cell-block technique for studying spleen tissue histology in dogs with ZVL. We examined 13 dogs with and seven dogs without Leishmania infantum infection. The dogs with Leishmania infection had a lower frequency of lymphoid follicles (2/13, Fisher's test, P<0.02) and a higher density of plasma cells (score 3, Fisher's test, P<0.02) than uninfected dogs (5/7 exhibiting lymphoid follicles and a plasma cell score of 1). The dogs with Leishmania infection also presented with granulomas (8/13) and infected macrophages (5/13). These differences in the histological presentations of spleen tissue from infected and uninfected dogs corresponded to changes observed in conventional histology. Hence, the cell-block technique described here may be used in the follow-up care and study of dogs with ZVL and other diseases in both clinical practice and research.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/27638124/