Peer-reviewed veterinary case report
A novel diagnostic platform based on multiplex ligase detection-PCR and microarray for simultaneous detection of swine viruses.
- Journal:
- Journal of virological methods
- Year:
- 2011
- Authors:
- Jiang, Yonghou et al.
- Affiliation:
- College of Life Sciences · China
Plain-English summary
Researchers have developed a new testing method that can quickly identify several viruses that affect pigs, particularly those causing reproductive and breathing issues. This method uses a combination of techniques to detect even small amounts of viral material, making it very sensitive and specific. In tests with clinical samples, the new method showed excellent accuracy, correctly identifying the viruses in nearly all cases. This approach could be very useful for veterinarians in diagnosing multiple infections in pigs at once. Overall, the new diagnostic tool appears to work very well.
Abstract
Simultaneous detection and identification of multiple pathogens is required in many diagnostic fields. In this study a novel method based on a multiplex ligase detection (LD)-polymerase chain reaction (PCR) and microarray (MLPM) is described to detect simultaneously several swine viruses involved in reproductive and/or respiratory problems. The multiplex diagnostic system was validated using standard plasmids, and clinical samples. Using this strategy as few as 10 copies of target plasmids were detected successfully. Each probe pair yielded specific positive signal only in its target site. In addition, when six target plasmids were present simultaneously sufficient robust signals were generated in their corresponding sites of six plasmid templates and no obvious signals were detected in non-target sites. Compared to real-time PCR, the MLPM showed specificities and sensitivities of 95.7-100% and 100% for 47 clinical samples tested, respectively. The results demonstrate that this novel assay is a specific, sensitive, and multiplex diagnostic method for detection of multiple pathogens and can also be adapted easily for diagnostic purposes.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/21951634/