Peer-reviewed veterinary case report
Measuring kidney injury markers in dogs after blood flow loss
By Davis, Jennifer et al.·Published in Research in veterinary science·2021·School of Veterinary Medicine, Australia·View original on PubMed →
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Original publication title: Ability of different assay platforms to measure renal biomarker concentrations during ischaemia-reperfusion acute kidney injury in dogs.
- Species:
- dog
Plain-English summary
Six male greyhounds underwent a procedure that caused temporary kidney damage, and researchers wanted to see how well different tests could measure kidney injury markers in their blood and urine. After the dogs experienced a period of reduced blood flow followed by treatment, only one marker, NGAL, showed a significant increase across all tests. The other markers' results varied depending on the testing method used, with some showing much lower levels than expected. This study suggests that more work is needed to ensure these tests can accurately detect kidney injury in dogs.
People also search for: dog kidney injury symptoms · greyhound acute kidney injury · NGAL test for dogs · kidney disease tests for dogs
Abstract
Several protein biomarkers have been shown to be useful for the early diagnosis of acute kidney injury (AKI) in animals and people. Multiplex assays for measurement of a panel of renal biomarkers in canine samples have recently become available. This study compared the use of two such assays, versus previously validated ELISAs, to measure five biomarkers in canine samples during ischaemia-reperfusion (IR) AKI. Blood and urine was collected from six male anaesthetised greyhounds that underwent 1-h of renal ischaemia (severe hypotension induced by acute haemorrhage) and 2-h of reperfusion (intravenous fluid resuscitation). Histology confirmed presence of acute tubular injury at 2 h of reperfusion. Concentrations of clusterin, cystatin C, kidney-injury molecule 1 (KIM-1), monocyte chemoattractant protein 1, and neutrophil gelatinase-associated lipocalin (NGAL) at baseline and following IR, measured by two different multiplex assays and previously-validated single analyte immunoassays, were compared. Only NGAL was significantly elevated following IR with all assays investigated. Whether concentrations of the other four biomarkers were significantly increased following IR depended on the assay used. Concentrations of cystatin C and KIM-1 measured with the multiplex assays were of a vast magnitude lower than those measured with the corresponding single analyte ELISAs. We conclude that further validation is required before these assays can reliably be used to measure AKI biomarkers in canine samples.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/33223120/