Acute lung injury and repair induced by single exposure ofin immunocompetent mice.

Abstract

Characterize the course of acutelung infection in immunocompetent mice, investigating the immunological, pathological and tissue functional modifications.C57BL/6 mice were intranasally infected withconidia and euthanized to access inflammatory parameters.Mice infected withshowed an inoculum-dependent lethality and body weight loss. An intense proinflammatory cytokine release, neutrophil infiltrate and pulmonary dysfunction was also observed in the early phase of infection. In the late phase of infection, proresolving mediators release, apoptosis and efferocytosis increased and lung tissue architecture is restored.Our study characterized an immunocompetent model of acute pulmonaryinfection in mice and opened an array of possibilities for investigations on interactions ofwith host-immune system.