Peer-reviewed veterinary case report
Amperometric immunosensor for diagnosis of BLV infection.
- Journal:
- Biosensors & bioelectronics
- Year:
- 2008
- Authors:
- Kurtinaitiene, Bogumila et al.
- Affiliation:
- Institute of Biochemistry
Abstract
A new amperometric immunosensor for detection of antibodies against bovine leukemia protein (gp51) was designed. The detection of antibody-antigen complex formation was based on application of secondary antibodies labeled with horseradish peroxidase (HRP). Ferrocenecarboxylic acid (FCA) and N,N,N',N'-tetramethylbenzidine (TMB) were selected as suitable mediators for this immunosensor. Optimal conditions for amperometric detection were found. Sensitivity of created system was compared with the results of enzyme-linked immunosorbent assay (ELISA) and agar gel immunodiffusion (AGID) reaction, and was sufficient for detection of usual anti-gp51 antibody concentration present in the blood serum of BLV-infected cattle.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/18294837/