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Peer-reviewed veterinary case report

Oral DNA vaccine using E. coli triggers immune response to parvovirus

By Dahiya, S S et al.·Published in Acta virologica·2011·Indian Veterinary Research Institute, India·View original on PubMed

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Original publication title: An oral Sindbis virus replicon-based DNA vaccine containing VP2 gene of canine parvovirus delivered by Escherichia coli elicits immune responses in dogs.

Species:
dog

Plain-English summary

A group of dogs received an experimental oral vaccine designed to protect against canine parvovirus (CPV), a serious virus that can cause severe gastrointestinal issues. After vaccination, the dogs showed a strong immune response, producing specific antibodies that can help fight off CPV. This study suggests that this new oral vaccine could be a promising option for vaccinating dogs against this dangerous virus.

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Abstract

A Sindbis virus replicon-based DNA vaccine containing VP2 gene of canine parvovirus (CPV) was delivered by Escherichia coli to elicit immune responses. The orally immunized dogs developed CPV-specific serum IgG and virus neutralizing antibody responses. The cellular immune responses analyzed using lymphocyte proliferation test and flow cytometry indicated CPV-specific sensitization of both CD3+CD4+ and CD3+CD8+ lymphocytes. This study demonstrated that the oral CPV DNA vaccine delivered by E. coli can be considered as a promising approach for vaccination of dogs against CPV.

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Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/22149493/