Analysis of paraoxonase-1 activity and cupric reducing antioxidant capacity in cerebrospinal fluid of dogs with neurological disorders.

Abstract

Cerebrospinal fluid (CSF) is a liquid that remains in close contact with the central nervous system (CNS) and is therefore commonly used to identify pathological alterations related to CNS diseases. However, sometimes techniques usually employed in clinical practice do not reveal alterations and other parameters, such as paraoxonase-1 (PON1) activity and total antioxidant capacity (TAC), are needed in this field. In this paper PON1 activity and TAC estimated by the cupric reducing antioxidant capacity (CUPRAC) method are analyzed in CSF of dogs with idiopathic epilepsy (IE), intracranial tumors (IT), inflammatory-based pathologies of the CNS (INF) as well as acute (AH) and chronic intervertebral disc herniations (CH). Analyses were performed within a maximum of three months after sample collection. No differences in CUPRAC values were found between the groups; however, the results revealed significantly higher values of PON1 activity in INF group compared to IE (P = 0.019), IT (P = 0.004) and AH (P = 0.038). Since the estimation of PON1 activity in CSF appears to be a potentially useful tool in canine neurology, future studies are needed to elucidate its role in clinical practice.