Peer-reviewed veterinary case report
Validation of a urine test measuring five kidney injury markers
By Davis, Jennifer et al.·Published in Research in veterinary science·2021·School of Veterinary Medicine, Australia·View original on PubMed →
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Original publication title: Analytical validation and reference intervals for a commercial multiplex assay to measure five novel biomarkers for acute kidney injury in canine urine.
- Species:
- dog
Plain-English summary
A study looked at how well a new urine test could measure five different markers for acute kidney injury (AKI) in dogs. The test was found to work accurately for one marker, NGAL, but other factors like bilirubin and hemoglobin in the urine could interfere with the results. The researchers also found that urine samples could be stored safely for a certain time without losing accuracy. Unfortunately, they couldn't establish normal ranges for one of the markers due to inconsistent results. This information could help vets better diagnose kidney issues in dogs using urine tests.
People also search for: dog kidney injury symptoms · urine test for dog kidney disease · elevated NGAL in dog urine
Abstract
Novel urinary biomarkers are increasingly utilized for the diagnosis of acute kidney injury (AKI) in dogs. Magnetic-bead based immunoassays for the simultaneous measurement of multiple biomarkers represent a potentially efficient and cost effective tool for investigators; however there is limited data to support their reliable use in dogs. Analytical validation of a commercial multiplex assay for the measurement of five AKI biomarkers: clusterin, cystatin C, kidney-injury molecule 1 (KIM-1), monocyte chemoattractant protein 1 and neutrophil gelatinase-associated lipocalin (NGAL) in canine urine was performed. The effect of pre-analytical factors including potential interfering substances and sample storage methods were investigated. Urine from 110 healthy dogs was used to determine reference intervals for each biomarker measured, according to American Society of Veterinary Clinical Pathology guidelines. Additionally, urine from 21 dogs with pyuria was used to evaluate the impact of pyuria on biomarker concentration. The assay performed with acceptable accuracy and precision for the measurement of NGAL only. Clinically relevant urine concentrations of bilirubin, haemoglobin, and synthetic colloid solutions led to interference (mean percentage difference > +/- 15% compared to control) with measurement of all or some of the biomarkers. All biomarkers were stable in urine stored at 20-22 °C for 2 h, 4 °C for 12 h, or -20 °C for 6 months. Reference intervals could not be established for KIM-1 due to unacceptable measurement imprecision (intra- and inter assay coefficient of variation 45% and 20% respectively). Urine NGAL concentration was significantly elevated in pyuria (P < 0.001).
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/34261014/