Peer-reviewed veterinary case report
Markers Ki67 and AgNOR show equal cell growth in dog lymph node tests
By Bauer, Natali B et al.Ā·Published in Journal of veterinary internal medicineĀ·2007Ā·Clinic of Small Animals, GermanyĀ·View original on PubMed ā
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Original publication title: Argyrophilic nucleolar organizing regions and Ki67 equally reflect proliferation in fine needle aspirates of normal, hyperplastic, inflamed, and neoplastic canine lymph nodes (n = 101).
- Species:
- dog
Plain-English summary
A study looked at 101 dogs with different lymph node conditions, including normal, inflamed, and lymphoma (a type of cancer). Researchers used special stains to count certain cells that indicate how fast the cells are growing. They found that dogs with lymphoma had significantly higher counts of these cells compared to the others, which helps veterinarians diagnose this condition. Both the AgNOR count and Ki67 percentage were effective in identifying lymphoma, with high accuracy rates. This means that if your dog has swollen lymph nodes, these tests can provide important information for diagnosis.
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Abstract
BACKGROUND: The count of argyrophilic nucleolar organizing regions (AgNOR) has been considered a useful variable that reflects cellular proliferation in canine lymph nodes, but it has not been compared with other markers of proliferation. HYPOTHESIS: Ki67 and AgNORs are equally useful as markers of tissue proliferation in fine needle aspirates of canine lymph nodes. ANIMALS: A total of 101 dogs. MATERIAL AND METHODS: Prospective, observational study of a convenience sample of dogs. Two smears were prepared for a May-Gruenwald-Giemsa stain and a Ki67/AgNOR double stain. In addition, CD3/CD79a immunostaining was performed when cytologic examination revealed a lymphoma. The dogs were grouped as normal (n = 26), reactive hyperplasia (n = 25), lymphadenitis (n = 31), and lymphoma (n = 19), based on the physical examination and the cytologic findings. The AgNOR count/cell, AgNOR area/cell and the percentage of cells staining positive for Ki67 were evaluated in 100-167 cells (median, 113 cells) by using automatic image analysis. RESULTS: Mean (SD) AgNOR counts/cell were 1.36 +/- 0.19 in normal dogs, 1.55 +/- 0.26 in lymphadenitis, 1.65 +/- 0.32 in reactive hyperplasia, and 3.67 +/- 1.08 in lymphoma. The percentage of Ki67 positive cells was 2.67 +/- 0.99% in normal lymph nodes, 5.04 +/- 3.34% in lymphadenitis, 5.36 +/- 2.14% in reactive hyperplasia, and 30.2 +/- 10.8% in lymphoma. All variables were significantly higher in dogs with lymphoma compared with the other groups (P < .0001). The sensitivity and the specificity of the AgNOR count for diagnosing lymphoma were 95 and 96% at a cutoff value of >2.04 AgNORs/cell. The cutoff value for the Ki67 positive cells was >10.40% (sensitivity, 95%; specificity, 98%). CONCLUSION AND CLINICAL IMPORTANCE: The results indicated that both AgNOR and Ki67 counts were good diagnostic tools for assessment of proliferation in aspirates of canine lymph nodes.
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Search related cases āOriginal publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/17939545/