Peer-reviewed veterinary case report
Best blood test for diagnosing canine visceral leishmaniasis in Rio
By da Silva, Denise Amaro et al.·Published in Veterinary journal (London, England : 1997)·2013·Pó, Brazil·View original on PubMed →
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Original publication title: Assessment of serological tests for the diagnosis of canine visceral leishmaniasis.
- Species:
- dog
Plain-English summary
A group of dogs in Rio de Janeiro were tested for canine visceral leishmaniasis, a serious disease caused by parasites spread by sandflies. Different blood tests were used, including an ELISA test, which showed the highest accuracy in diagnosing the disease. The ELISA-L. chagasi test was the most reliable, but a simpler test called the immunochromatographic test also showed promise for quick results without needing a lab. Early diagnosis is crucial for effective treatment, so these tests can help veterinarians identify the disease sooner and start appropriate care.
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Abstract
An immunoenzymatic assay (ELISA), an indirect immunofluorescence antibody test (IFAT) with different antigens (ELISA-Leishmania chagasi, ELISA-L. major-like, IFAT-L. chagasi and IFAT-L. major-like), and an immunochromatographic test were assessed for the diagnosis of canine visceral leishmaniasis (CVL). Serum samples from 144 dogs from an endemic area for visceral leishmaniasis in the municipality of Rio de Janeiro were tested. The sensitivities of the serological tests were 93%, 100%, 73%, 60% and 93%, with specificities of 87%, 92%, 77%, 96% and 92% for the ELISA-L. major-like, ELISA-L. chagasi, IFAT-L. major-like, IFAT-L. chagasi and the immuno chromatographic test, respectively. ELISA-L. chagasi was the best test for the diagnosis of CVL, but the immunochromatographic test could be a useful alternative as it offers simple and rapid diagnosis without the need for a specialized laboratory.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/22789627/