Peer-reviewed veterinary case report
B-prolymphocytic leukemia cells that form rosettes with sheep red blood cells through monoclonal surface immunoglobulin.
- Journal:
- American journal of hematology
- Year:
- 1985
- Authors:
- Maruta, A et al.
Plain-English summary
In a study involving a patient with B-cell prolymphocytic leukemia, researchers found that the cancerous cells could form clusters, known as rosettes, with sheep red blood cells. Tests showed that these cells did not react to certain T-cell markers, but they did show features typical of B-cells, including specific proteins on their surface. The formation of these rosettes was linked to the binding of these surface proteins to the sheep red blood cells. Additionally, it appeared that the patient's blood contained antibodies that could cause the sheep red blood cells to clump together. The study suggests that the leukemia cells have unique properties that could be important for understanding this type of cancer.
Abstract
The neoplastic cells from a patient with B-cell prolymphocytic leukemia exhibited sheep red blood cell (SRBC) rosette formation. Immunochemistries revealed no reactivity with T-cell monoclonal antibodies OKT 3, 4, 8, and 11. However, the neoplastic cells expressed characteristics of B-cells including surface IgM and IgD associated with lambda light chains, Ia-like antigenicity, and reactivity to monoclonal antibodies against B-cell antigens B1, B2, and B4. Inhibition procedures revealed that SRBC rosette formation was the result of binding activity of surface immunoglobulin to SRBC. Pretreatment of the leukemic cells with antihuman IgM or lambda antisera, or pronase or trypsin blocked rosette formation with SRBC. Circulating antibody to SRBC was also suspected by the fact that preincubation of SRBC with heat-inactivated patient's serum resulted in agglutination of the SRBC.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/3877461/