Peer-reviewed veterinary case report
BLV-CoCoMo-qPCR-2: improvements to the BLV-CoCoMo-qPCR assay for bovine leukemia virus by reducing primer degeneracy and constructing an optimal standard curve.
- Journal:
- Archives of virology
- Year:
- 2015
- Authors:
- Takeshima, Shin-nosuke et al.
- Affiliation:
- Viral Infectious Diseases Unit · Japan
Plain-English summary
Bovine leukemia virus (BLV) is a virus that causes a serious disease in cattle known as enzootic bovine leukosis, which is the most common type of cancer in these animals. Sometimes, cows infected with BLV don't show any signs of illness, so measuring the amount of virus in their blood is important for understanding how the disease is progressing. Researchers improved a testing method called CoCoMo-qPCR to create a new version called "BLV-CoCoMo-qPCR-2," which is better at detecting the virus by using specially designed components. They also made a new standard for comparison and tested samples from infected cows using both methods to see how well they worked.
Abstract
Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis, which is the most common neoplastic disease of cattle. Because BLV infection can remain clinically silent, the proviral load is an important index for estimating disease progression. CoCoMo-qPCR, an assay developed to estimate BLV proviral load, allows the highly sensitive detection of BLV originating in different countries. Here, we developed a modified version of the CoCoMo-qPCR assay, the "BLV-CoCoMo-qPCR-2" assay, which uses optimized degenerate primers. We also constructed a new plasmid standard. Finally, we used both assays to examine DNA samples from BLV-infected cattle and compared the results.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/25731158/