Peer-reviewed veterinary case report
How fine-needle biopsy helps diagnose lymphoma in dogs
By Caniatti, M. et al.·Published in Veterinary Pathology·1996·Istituto di Anatomia Patologica Veterinaria e Patologia Aviare·View original on Crossref →
PetCaseFinder translated the abstract of this peer-reviewed paper into plain English so pet owners can read it. We do not publish original research — every detail traces back to the citation above. How we work →
Original publication title: Canine Lymphoma: Immunocytochemical Analysis of Fine-needle Aspiration Biopsy
- Species:
- dog
Plain-English summary
A group of dogs with swollen lymph nodes was tested for lymphoma, a type of cancer that affects the immune system. The tests involved taking samples from the enlarged lymph nodes and analyzing them to determine the type of lymphoma present. Out of 21 dogs, 14 were diagnosed with B-cell lymphoma and 3 with T-cell lymphoma, while one case was classified as a non-B-non-T lymphoma. The study found that using specific antibodies in the analysis helped accurately identify the type of lymphoma. This method proved to be effective for diagnosing and classifying the disease in dogs.
People also search for: dog swollen lymph nodes lymphoma · canine lymphoma diagnosis · dog lymph node cancer treatment
Abstract
Cytospin preparations of fine-needle aspirates from 21 dogs with peripheral lymphadenopathy (18 with lymphoma and three with lymph node hyperplasia) were studied by combining morphologic and immunocytochemical analysis. Fine-needle aspirates were taken from at least two enlarged lymph nodes, and the diagnosis was based on air-dried smears stained with May-Grünwald Giemsa. Fine-needle aspiration biopsy always provided an adequate quality and quantity of cells to perform morphologic and immunologic studies. Immunophenotyping was performed on cytospin preparations with a panel of eight monoclonal antibodies specific for canine cell surface antigens and one rabbit polyclonal antibody (A452) against human CD3, which cross-reacts with dog antigen. The immunocytochemical study resulted in the diagnosis of 14 B-cell lymphomas (CD21 +, CD3-) and three T-cell lymphomas (all CD3 +, two CD8+). One lymphoma lacked surface antigens specific for the B- or T-cell lineage and was classified as non-B-non-T lymphoma (CD21-, CD3-, CD4-, CD8-). The monoclonal antibodies CA12.10C12, CA4.1D3, and CA1D6 and the polyclonal antibody A452, used as a group, appeared to be the most useful reagents to suggest lymphoid origin and to discriminate between T- and B-cell phenotype. Cytospin preparations in combination with immunocytochemistry provided a practical, economical, and accurate method for the diagnosis and phenotyping of canine lymphoma.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication on Crossref: https://doi.org/10.1177/030098589603300210