Peer-reviewed veterinary case report
Flow cytometry test for diagnosing canine visceral leishmaniasis
By A.V. Carvalho Neta et al.·Published in Arquivo Brasileiro de Medicina Veterinária e Zootecnia·2006·View original on DOAJ →
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Original publication title: Citometria de fluxo no diagnóstico da leishmaniose visceral canina Flow cytometry used in canine visceral leishmaniasis diagnosis
- Species:
- dog
Plain-English summary
A group of dogs was tested for canine visceral leishmaniasis (a serious disease caused by a parasite) using a new blood test method called AAPF-IgG. This method was able to accurately identify dogs that were infected with the parasite, distinguishing 96% of infected dogs from healthy ones. The test showed promising results, making it a potential new tool for veterinarians to diagnose this disease more effectively. If you suspect your dog may have leishmaniasis, discussing this new testing method with your vet could be beneficial.
People also search for: dog leishmaniasis symptoms · canine visceral leishmaniasis treatment · blood test for dog parasites
Abstract
Descreve-se a padronização de nova metodologia para detecção de anticorpos antiformas promastigotas fixadas de L. (L.) chagasi, por citometria de fluxo (AAPF-IgG), sua aplicabilidade e desempenho na identificação de casos de leishmaniose visceral canina (LVC). Foram avaliados dois grupos de cães classificados pela reação de imunofluorescência indireta (RIFI), como: não reatores (NR, n=10) e reatores (R, n=50) dos quais foram coletadas amostras de sangue (soro) para realização dos testes laboratoriais. Os resultados relacionados ao estabelecimento, aplicabilidade e desempenho da metodologia AAPF-IgG demonstraram que essa metodologia possibilita a identificação de uma região de reatividade diferencial entre cães NR e R, no soro diluído a 1:2048 e o valor de 20% de parasitos fluorescentes positivos (PPFP) como ponto de corte entre resultados positivos e negativos, mostrando que a AAPF-IgG aplica-se na identificação de casos de LVC, possibilitando distinguir 96% de cães R como positivos e 100% de cães NR como negativos. Esses resultados em conjunto sugerem que a utilização da AAPF-IgG pode ser um novo instrumento para ensaios clínicos de diagnóstico sorológico da LVC.<br>The current study evaluated the standardization of a new methodology for detection of anti-fixed L. (L.) chagasi promastigote antibodies by flow cytometry (AAPF-IgG), as well its applicability and performance in the identification of cases of Canine Visceral Leishmaniasis (CVL). Two groups of dogs were classified by RIFI (gold standard) as no reactors (NR, n=10) and reactors (R, n=50). Blood samples were collected and used for the laboratorial tests (RIFI and AAPF-IgG). The results showed that the new AAPF-IgG assay makes possible the identification of an area of differential reactivity between dogs NR and R at the dilution of 1:2048 and 20% of percentage of positive fluorescent parasite as the cut point among positive and negative results. The AAPF-IgG assay was able to distinguish 96% of R dogs as positive and 100% of NR dogs as negative. Hence, those data support the applicability of flow cytometry AAPF-IgG method as a new instrument for serological diagnosis of CVL.
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Search related cases →Original publication on DOAJ: https://doi.org/10.1590/S0102-09352006000400005