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Peer-reviewed veterinary case report

Combination of an unbiased amplification method and a resequencing microarray for detecting and genotyping equine arteritis virus.

Journal:
Journal of clinical microbiology
Year:
2015
Authors:
Hans, Aymeric et al.
Affiliation:
ANSES-Dozul&#xe9 · France
Species:
horse

Abstract

This study shows that an unbiased amplification method applied to equine arteritis virus RNA significantly improves the sensitivity of the real-time reverse transcription-quantitative PCR (RT-qPCR) recommended by the World Organization for Animal Health. Twelve viral RNAs amplified using this method were hybridized on a high-density resequencing microarray for effective viral characterization.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/25339390/