Peer-reviewed veterinary case report
How PCR tests find parvovirus in puppies under 3 months
By Sibel Hasırcıoğlu & Hatice Pelin Aslım·Published in Turkish Journal of Agriculture: Food Science and Technology·2022·Department of Virology, Faculty of Veterinary, University of Selcuk, 42130 Konya, TR·View original on DOAJ →
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Original publication title: Comparative Detection of Canine Parvovirus by Differential PCR, Conventional PCR and Nested PCR tests: Detecting Antigenic Variants by Differential PCR
- Species:
- dog
Plain-English summary
A group of 35 puppies suspected of having parvovirus were tested for the virus using different lab methods. The results showed that 20 puppies tested positive with one method, while 31 tested positive with a more sensitive method. Parvovirus is a serious illness that can cause severe gastrointestinal problems in young dogs, especially in males and those under three months old. It's important for pet owners to be aware that even vaccinated puppies can sometimes get this disease. Early detection and proper testing are crucial for managing parvovirus effectively.
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Abstract
Canine parvovirus (CPV) is a pathogen causing hemorrhagic enteritis in puppies and mainly transmitting via feco-oral route. In this study, stool samples were collected from a total of 35 animals suspected of CPV. The samples were examined by Conventional PCR, Nested PCR and Differential PCR tests. 20 out of 35 dogs (57.1%) were detected positive by conventional PCR, 31 (88.6%) by nested PCR and 30 (85.7%) by differential PCR. CPV 2a was stated as the most common antigenic type, male animals and 0–3-month-olds had a high rate of becoming sick and vaccinated animals might also catch the disease, rarely. Accordingly, it is recommended to focus on studies providing molecular epidemiology surveillance in order to detect the existing subtypes and develop reliable diagnosis and vaccination methods.
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Search related cases →Original publication on DOAJ: https://doi.org/10.24925/turjaf.v10i2.254-259.4653