Peer-reviewed veterinary case report
Best tests and signs to diagnose leishmaniasis in dogs
By María Paz Peris et al.·Published in Microorganisms·2021·Animal Pathology Department, Veterinary Faculty, University of Zaragoza, 50013 Zaragoza, Spain, CH·View original on DOAJ →
PetCaseFinder translated the abstract of this peer-reviewed paper into plain English so pet owners can read it. We do not publish original research — every detail traces back to the citation above. How we work →
Original publication title: Comparative Study of Real-Time PCR (TaqMan Probe and Sybr Green), Serological Techniques (ELISA, IFA and DAT) and Clinical Signs Evaluation, for the Diagnosis of Canine Leishmaniasis in Experimentally Infected Dogs
- Species:
- dog
Plain-English summary
A group of dogs infected with the parasite that causes canine leishmaniasis (CanL) underwent various tests to find the best way to diagnose the disease. Researchers found that using a simple conjunctival swab (a sample taken from the eye area) combined with advanced DNA testing methods (qPCR) was effective in detecting the infection. This approach could help avoid more invasive procedures like taking samples from bone marrow or lymph nodes. The study suggests that this method could reliably identify both early and late stages of the disease in dogs.
People also search for: dog leishmaniasis symptoms · conjunctival swab for dog disease · canine leishmaniasis treatment options
Abstract
Canine leishmaniasis (CanL) diagnosis is not fully resolved. Currently, two specific methodologies are in continuous development, the detection of the parasite DNA or RNA in target organs and the detection of specific antibodies against <i>Leishmania</i> sp. For a correct diagnosis, it has been shown that the joint use of this type of test is necessary. In this work, a Sybr Green and a TaqMan Probe based on real time PCRs (qPCR) was performed for the detection of <i>Leishmania</i> sp. in order to correlate the results with clinicopathological and serological evaluations (IFA, ELISA and DAT) to propose an optimal biological sample to be used to detect the parasite in both early and late stages of the infection. A total of four samples were processed: conjunctival swabs, popliteal lymph node aspirates, bone marrow aspirates, and peripheral blood from experimentally infected dogs belonging to a larger study. Our results indicated that a single non-invasive sample (conjunctival swab) and the application of both types of qPCR would be reliable for determining <i>Leishmania</i> infection as well as the disease stage in dogs, thus avoiding bone marrow, lymph node aspirate or blood samples collection.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication on DOAJ: https://doi.org/10.3390/microorganisms9122627