Peer-reviewed veterinary case report
Detecting Leishmania antibodies in dogs with rapid and lab tests
By Villanueva-Saz, Sergio et al.·Published in Acta veterinaria Scandinavica·2019·Department of Pharmacology and Physiology, Spain·View original on PubMed →
PetCaseFinder translated the abstract of this peer-reviewed paper into plain English so pet owners can read it. We do not publish original research — every detail traces back to the citation above. How we work →
Original publication title: Comparison of a qualitative immunochromatographic test with two quantitative serological assays for the detection of antibodies to Leishmania infantum in dogs.
- Species:
- dog
Plain-English summary
A study evaluated a new rapid test for detecting Leishmania infantum, a parasite that can cause serious health issues in dogs. The test was compared to two established methods for checking if dogs had been infected. It showed excellent results, correctly identifying all infected dogs and almost all healthy ones. This means that the rapid test can be a reliable option for vets to quickly determine if a dog has this infection, which is important for both the dog's health and preventing the spread to humans.
People also search for: dog leishmaniasis test · rapid test for Leishmania in dogs · symptoms of Leishmania infection in dogs
Abstract
Canine leishmaniosis is a disease caused by Leishmania infantum, a vector-borne parasite. Due to the zoonotic potential of canine leishmaniosis, infected dogs must be identified. Serological assays are the most common methods for the detection of L. infantum infection in dogs used in veterinary practice. The aim of the study was to assess the performance of a rapid immunochromatographic test (FASTest LEISH, MEGACOR Diagnostik) for the detection of specific antibodies to that of the L. infantum in dog sera. The results were simultaneously compared using a commercial brand of indirect immunofluorescence antibody test and an in-house enzyme-linked immunosorbent assay as references. Between the two reference tests, 232 serum samples out of 244, produced concordant results while 12 exhibited discordant results. Of the 232 concordant samples, 121 were classified as L. infantum seropositive, and 111 samples were previously classified as L. infantum seronegative by a combination of the reference assays. All samples that were seropositive by the reference tests were also positive according to the rapid test, and only one sample that was seronegative according to the two reference assays was positive according to the rapid test. Compared with the reference tests, the rapid test sensitivity was 100%, specificity was 99.1%, accuracy was 99.6%, Cohen's kappa coefficient was 0.99, and the area under receiver operating characteristic curve was 0.995. The FASTest LEISHis a rapid, qualitative in-clinic test with high sensitivity and specificity.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/31391084/