Peer-reviewed veterinary case report
Comparison of primer sets and one-step reverse transcription polymerase chain reaction kits for the detection of bluetongue viral RNA.
- Journal:
- Journal of virological methods
- Year:
- 2014
- Authors:
- Lee, Fan et al.
- Affiliation:
- Animal Health Research Institute
Abstract
Bluetongue virus is the etiological agent of bluetongue, one of the most important insect-transmitted animal diseases in the world. To establish a feasible diagnostic procedure for detecting the viral RNA, seven commercially available one-step RT-PCR kits in combination with three primer sets were evaluated. Results of this study showed remarkable differences in analytical sensitivity between the examined RT-PCR kits. In addition, it was found that a World Organization for Animal Health-recommended primer set may not be effective in detecting most BTV RNA.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/24503041/