Peer-reviewed veterinary case report
Best lab tests to diagnose parvovirus infection in puppies
By M.M.O. Silva et al.·Published in Arquivo Brasileiro de Medicina Veterinária e Zootecnia·2013·Universidade Federal Fluminense, BR·View original on DOAJ →
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Original publication title: Comparison of three laboratorial tests for diagnosis of canine parvovirus infection
- Species:
- dog
Plain-English summary
A group of puppies with diarrhea were tested for canine parvovirus (CPV) using three different lab tests to see which was most effective. The enzyme immunoassay (EIA) detected the virus in 44 samples, while the hemagglutination test (HA) found it in 32 samples, and the polymerase chain reaction (PCR) test identified 57 positive cases, including some that the other tests missed. The EIA and PCR tests showed the best agreement, meaning they often gave similar results. This study suggests that if the EIA or HA tests come back negative, it's important to follow up with PCR to ensure an accurate diagnosis.
People also search for: puppy diarrhea parvovirus test · canine parvovirus symptoms · best test for dog parvovirus
Abstract
The aim of this study was to evaluate the rapid tests currently used for canine parvovirus (CPV) diagnosis: hemagglutination test (HA), enzyme immunoassay (EIA) and polymerase chain reaction (PCR). A total of 112 fecal samples collected from diarrheic puppies up to one year of age were tested. The EIA was able to detect CPV antigen in 44 samples. By HA, 32 samples tested highly positive with titers >128, eight tested weakly positive (titers 32 and 64) and 72 were negative (titers <16). Using PCR, 57 samples were found positive including 13 EIA-negative and 19 HA-negative samples. The best correlation was observed between EIA and PCR (88.4%). These tests were able to detect all types of CPV, including CPV-2c. Considering that 23%-33% of dogs presenting enteritis did not show infection by EIA nor HA, negative results from the antigen detection tests should be confirmed through molecular methods.
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Search related cases →Original publication on DOAJ: https://doi.org/10.1590/S0102-09352013000100023