Peer-reviewed veterinary case report
Multicolor GeneScan test detects immune gene changes in dog lymphoid
By Goto-Koshino, Yuko et al.Ā·Published in Veterinary immunology and immunopathologyĀ·2015Ā·The University of Tokyo, JapanĀ·View original on PubMed ā
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Original publication title: Construction of a multicolor GeneScan analytical system to detect clonal rearrangements of immunoglobulin and T cell receptor genes in canine lymphoid tumors.
- Species:
- dog
Plain-English summary
A group of dogs with lymphoid tumors, including multicentric lymphoma and gastrointestinal lymphoma, underwent a new testing method to detect specific gene rearrangements. This advanced technique, using a multicolor GeneScan system, successfully identified clonal rearrangements in over 93% of dogs with multicentric lymphoma and 84% with gastrointestinal lymphoma. The method improved the sensitivity of detection, allowing for the identification of abnormal cells even when they were present in small amounts. This innovative approach could enhance the diagnosis of lymphoid tumors in dogs, helping veterinarians provide better treatment options.
People also search for: dog lymphoma symptoms Ā· canine cancer diagnosis Ā· dog gene testing for lymphoma
Abstract
Polymerase chain reaction (PCR) amplification to detect immunoglobulin heavy chain (IgH) and T cell receptor γ-chain (TCRγ) gene rearrangements has recently become widely used as part of the diagnostic strategy for lymphoid tumors in dogs. In this study, we constructed a multicolor GeneScan analytical system to improve the sensitivity and resolution of the clonality analysis of antigen receptor gene rearrangements in dogs. We used 7 reactions per sample, with 2 PCR conditions, to amplify IgH/TCRγ and control genes. By using multicolor-labeled primers, these 7 PCR products could be combined into 3 tubes before capillary electrophoresis. Clonal rearrangement of the IgH/TCRγ genes was detected in 93.3% of dogs with multicentric lymphoma and 84.6% of dogs with gastrointestinal lymphoma. Detection sensitivity of the clonally expanded cells in the background of normal peripheral blood mononuclear cells was 1-10%. The multicolor GeneScan analytical system developed here may prove to be helpful for the diagnosis of lymphoid tumors in dogs.
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Search related cases āOriginal publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/25840823/