Peer-reviewed veterinary case report
Cyclin D1 protein and gene changes in dog mouth melanoma tumors
By Zamboni, Clarissa et al.·Published in Veterinary and comparative oncology·2020·Department of Comparative Biomedicine and Food Science, Italy·View original on PubMed →
PetCaseFinder translated the abstract of this peer-reviewed paper into plain English so pet owners can read it. We do not publish original research — every detail traces back to the citation above. How we work →
Original publication title: Cyclin D1 immunohistochemical expression and somatic mutations in canine oral melanoma.
- Species:
- dog
Plain-English summary
A study looked at 28 dogs with oral melanoma, a type of mouth tumor that is common in dogs. Researchers found that a protein called Cyclin D1 was present in about 69% of the tumors, while another marker, Ki-67, was found in 88.5% of cases. The presence of these markers could help predict how aggressive the tumor might be. Additionally, they discovered some genetic changes in the tumors, but these weren't consistent across all samples. More research is needed to understand the significance of these findings and how they might help in treating dogs with oral melanoma.
People also search for: dog oral melanoma treatment · Cyclin D1 in dog tumors · Ki-67 in canine cancer
Abstract
Canine oral melanoma (COM) is the most frequent tumour with oral localization in dogs. Copy number gains and amplifications of CCND1, a gene coding for Cyclin D1, are the most frequent chromosomal aberrations described in human non-UV induced melanomas. Twenty-eight cases of COM were retrieved from paraffin-blocks archives. A total of 4 μm thick sections were immunostained with an antibody against human Cyclin D1 and Ki-67. Cyclin D1 and Ki-67 expressions were scored through two counting methods. DNA was extracted from 20 μm thick sections of formalin-fixed paraffin-embedded blocks. Pathological and surrounding healthy tissue was extracted independently. Cyclin D1 immunolabelling was detected in 69% (18/26) while Ki-67 was present in 88.5% (23/26) of cases. Statistical analysis revealed correlation between two counting methods for Cyclin D1 (r = 0.54; P = .004) and Ki-67 (r = 0.56; P = .003). The correlation found between Ki-67 and Cyclin D1 indexes in 16/26 cases labelled by both antibodies (r = 0.7947; P = .0002) suggests a possible use of Cyclin D1 index as prognostic marker. Polymerase chain reaction analysis on CCND1 coding sequence revealed the presence of nine somatic mutations in seven samples producing synonymous, missense and stop codons. Since none of the single-nucleotide polymorphisms was found to be recurrent, it is suggested that overexpression of Cyclin D1 may be the consequence of alterations of CCND1 upstream regions or other genetic aberrations not detectable with the methodology used in this study. Future studies are needed to verify the potential use of Cyclin D1 index as prognostic indicator and to highlight the molecular events responsible for Cyclin D1 overexpression in COMs.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/31503380/