Peer-reviewed veterinary case report
Detection and Quantification of Transforming Growth Factor-β1 Produced by Murine B Cells: Pros and Cons of Different Techniques.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2021
- Authors:
- Mishima, Yoshiyuki et al.
- Affiliation:
- Department of Internal Medicine II · Japan
Abstract
Transforming growth factor (TGF)-β1 is one of the regulatory cytokines produced by B cells and has a pivotal role in intestinal homeostasis. TGF-β1 can determine the fate of naive T cells, such as differentiation, proliferation, and apoptosis, which are relevant to the pathogenesis of autoimmunity, infection, inflammation, allergy, and cancer. Here, we describe detailed methods for detection and quantification of TGF-β1 secreted by B cells using ELISA, flow cytometry, and real-time PCR.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/33479896/