Peer-reviewed veterinary case report
Detecting Anaplasma phagocytophilum infection in Brazilian dogs
By Santos, Huarrisson A et al.·Published in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·2011·Department of Animal Parasitology, Brazil·View original on PubMed →
PetCaseFinder translated the abstract of this peer-reviewed paper into plain English so pet owners can read it. We do not publish original research — every detail traces back to the citation above. How we work →
Original publication title: Detection of Anaplasma phagocytophilum in Brazilian dogs by real-time polymerase chain reaction.
- Species:
- dog
Plain-English summary
A group of dogs in Brazil tested positive for Anaplasma phagocytophilum, a bacteria that can cause illness in both dogs and humans. Out of 253 dogs sampled, 18 were found to have the infection, which is often spread by ticks. This finding suggests that dogs in these areas could potentially spread the bacteria to ticks, posing a risk to both pets and people. It's important for pet owners in these regions to be aware of tick prevention and to consult their veterinarian if their dog shows symptoms like fever or lethargy.
People also search for: dog tick disease symptoms · Anaplasma phagocytophilum in dogs · how to prevent ticks on dogs
Abstract
Anaplasma phagocytophilum was detected in dogs from Brazil in the municipalities of Seropédica and Itaguaí, Rio de Janeiro state, by real-time polymerase chain reaction (PCR) using SYBR Green to detect the amplification. Of 253 samples, 18 (7.11%) were positive, with a threshold cycle (Ct) ranging from 31 to 35 cycles. The PCR product from a positive sample was cloned and sequenced. The sequence obtained demonstrated 100% identity with other A. phagocytophilum sequences published in the GenBank database. The analytical sensitivity of RT-PCR using SYBR Green system was able to detect 3 plasmid copies when defined numbers of plasmid copies containing 122 base pairs from the msp2 gene were used. The assay was considered specific when DNA from bacteria (Anaplasma platys, Anaplasma marginale, Ehrlichia canis, Neorickettsia risticii, Rickettsia rickettsii) closely related to A. phagocytophilum was placed in the reaction. These results demonstrate that the canine granulocytic anaplasmosis agent is present in regions in which dogs could be a source of infection for tick vectors. The current study reports the detection of A. phagocytophilum, a zoonotic agent responsible for Human granulocytic anaplasmosis, in Brazilian dogs.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/21908321/