Peer-reviewed veterinary case report
Rapid test detects Anaplasma platys infection in dogs
By Li, Hua-tao et al.·Published in Veterinary journal (London, England : 1997)·2014·College of Veterinary Medicine, China·View original on PubMed →
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Original publication title: Detection of Anaplasma platys in dogs using real-time loop-mediated isothermal amplification.
- Species:
- dog
Plain-English summary
A dog with a low platelet count was tested for a parasite called Anaplasma platys, which can cause serious blood problems. Researchers developed a new, quick test that can detect this parasite in blood samples. The test showed similar accuracy to a more complex method but is faster and potentially cheaper. This new testing method could help veterinarians diagnose Anaplasma platys infections more easily and get dogs the treatment they need.
People also search for: dog low platelet count · Anaplasma platys symptoms · quick test for dog blood infection
Abstract
Anaplasma platys is a parasite of canine platelets that causes infectious cyclic thrombocytopenia. In this study, a novel real-time loop-mediated isothermal amplification (RT-LAMP) method was developed to detect A. platys. RT-LAMP primer sets were designed using a citrate synthase gene sequence and the assay was performed at 63 °C for 30 min. No cross-reactivity was observed with other Anaplasma or Ehrlichia spp. and the method exhibited a similar level of sensitivity in detecting the organism in 58 canine blood samples to that of a nested PCR. This RT-LAMP is a rapid and potentially cost-effective method of diagnosing A. platys infection in dogs.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/24508323/