Peer-reviewed veterinary case report
Detection of avian encephalomyelitis virus in chickens in Japan using RT-PCR.
- Journal:
- The Journal of veterinary medical science
- Year:
- 2019
- Authors:
- Goto, Yusuke et al.
- Affiliation:
- Iwate Prefecture Central Livestock Hygiene Service Center · Japan
- Species:
- bird
Abstract
A reverse transcription-polymerase chain reaction (RT-PCR) method was developed for broadly detecting the avian encephalomyelitis virus (AEV). The new primers were based on conserved sequences of the 5'-untranslated region of AEV, because the virus was not detected using previous reported RT-PCR. By applying this method to the chicken samples with suspected AEV infection in Japan, we successfully obtained PCR products of the predicted size from all samples, and we confirmed the presence of AEV via sequence analysis.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/30464075/