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Peer-reviewed veterinary case report

Detection of Babesia bigemina DNA in ticks by DNA hybridization using a nonradioactive probe generated by arbitrary PCR.

Journal:
Veterinary parasitology
Year:
2006
Authors:
Ravindran, Reghu et al.
Affiliation:
Indian Veterinary Research Institute · India

Abstract

The Dig-labeled probe specific to Babesia bigemina generated from monomorphic RAPD fragment of approximately 873 bp size amplified by a 10 mer CGGTGGCGAA, detected up to 100 ng of template DNA. This nonradioactive probe also detected B. bigemina in preparations of larval tick DNA from two of the five samples on dot-blot hybridization.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/16782278/