Peer-reviewed veterinary case report
How to detect cancer spread in bone marrow of dogs and cats?
By Taylor, Bonnie E et al.·Published in Veterinary clinical pathology·2013·Donaldson-Atwood Cancer Center, United States·View original on PubMed →
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Original publication title: Detection of carcinoma micrometastases in bone marrow of dogs and cats using conventional and cell block cytology.
- Species:
- dog
Plain-English summary
This study looked at whether a special test called cell block cytology could help find tiny cancer cells, known as micrometastases, in the bone marrow of dogs and cats with different types of cancer. Researchers examined the bone marrow of 13 dogs and 4 cats, using both routine tests and this new method. They found signs of micrometastases in two dogs with advanced cancer, and one of these cases showed specific markers that indicated the presence of cancer cells. The results suggest that cell block cytology is a helpful tool for checking bone marrow and can be used to identify these cancer cells effectively.
Abstract
BACKGROUND: The presence of human breast carcinoma micrometastases in bone marrow is associated with poor overall survival, poor breast-cancer-specific survival, poor disease-free survival, and poor distant disease-free survival. In veterinary practice, the detection of micrometastases as a component of clinical staging is a routine practice for lymphomas and mast cell tumors, but not for carcinomas. OBJECTIVES: This prospective study evaluated whether the identification of micrometastases from various carcinomas in dogs and cats in bone marrow using cell block cytology is technically feasible and whether it correlates with routine cytologic examination. METHODS: Thirteen dogs and 4 cats with various types of carcinomas were available for analysis. Routine and cell block cytologic evaluation combined with immunocytochemical staining with antibodies to CKAE1/AE3 and CK7 were performed on all bone marrow samples. RESULTS: Bone marrow micrometastasis was demonstrated by both methods in 2 dogs with advanced disease. In one case cells were immunoreactive for both CKAE1/AE3 and CK7. CONCLUSIONS: This study demonstrates that cell block cytology is a practical and useful method for bone marrow evaluation and is suitable for cytokeratin immunocytochemical analysis.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/23278287/