Peer-reviewed veterinary case report
Detection of influenza A virus by RT-PCR and standard methods in experimental infection of Ducks.
- Journal:
- The new microbiologica
- Year:
- 2005
- Authors:
- Foni, Emanuela et al.
- Affiliation:
- Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini" · Italy
Abstract
Cloacal swabs collected from mallard ducks (Anas platyrhynchos) experimentally infected with a H7N1 avian influenza strain were examined by Reverse Transcription Polymerase Chain Reaction to detect the influenza A virus. Reverse Transcription Polymerase Chain Reaction was compared with standard methods: inoculation of embryonated chicken eggs and inoculation of three established cell lines: Newborn Swine Kidney cells, Newborn Pig Trachea cells and Madine Darby Canine Kidney cells. Reverse Transcription Polymerase Chain Reaction was performed using a set of primers based on conserved regions of the matrix and nucleoprotein genes.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/15782624/