Peer-reviewed veterinary case report
How PCR testing helps tell dog intestinal lymphoma from enteritis
By Ohmura, S et al.·Published in Veterinary and comparative oncology·2017·Clinic of Small Animal Medicine, Germany·View original on PubMed →
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Original publication title: Detection of monoclonality in intestinal lymphoma with polymerase chain reaction for antigen receptor gene rearrangement analysis to differentiate from enteritis in dogs.
- Species:
- dog
Plain-English summary
A group of dogs with suspected intestinal issues, including 29 with intestinal lymphoma and 14 with enteritis (inflammation of the intestines), underwent testing to determine the cause of their symptoms. Researchers used a special test called polymerase chain reaction (PARR) to analyze tissue samples and found that 76% of the lymphoma cases showed a specific genetic pattern, while all enteritis cases had a different pattern. This testing helps veterinarians distinguish between intestinal lymphoma and enteritis, which can be challenging based on symptoms alone. The study suggests that PARR analysis is a valuable tool for accurate diagnosis in dogs with intestinal problems.
People also search for: dog intestinal lymphoma symptoms · enteritis in dogs treatment · how to diagnose dog lymphoma
Abstract
The diagnosis of canine intestinal lymphoma by morphological examination is challenging, especially when endoscopic tissue specimens are used. The utility of detection of antigen receptor gene rearrangement by polymerase chain reaction (PARR) in canine lymphoma has been well established, but its usefulness to distinguish enteritis and intestinal lymphoma remains unclear. In this retrospective study we assessed clonality of 29 primary canine intestinal lymphoma, 14 enteritis and 15 healthy control cases by PARR analysis, using formalin-fixed, paraffin-embedded full-thickness tissue specimens. We could detect monoclonal rearrangements in 22 of 29 canine intestinal lymphomas [76%; 95% confidence interval (CI) 56-90%] and polyclonal rearrangements in all of the enteritis and healthy control cases (100%; CI 88-100%). We revealed a predominance of T-cell phenotype compared to B-cell phenotype (85%; CI 65-96% and 15%; CI 4-35%, respectively). We showed that PARR analysis contributes to differentiation of canine intestinal lymphoma from enteritis and to phenotyping of lymphomas.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/25988583/