Detection offrom rodents residing in the densely populated residential regions along the coastal areas of Banyuwangi Sub District, Indonesia.

Abstract

BACKGROUND: Extensive attention has been devoted to studies ofin rodents ever since it became recognised as a zoonotic pathogen known as atypical human trypanosomiasis. Regrettably, althoughinfections of small mammals remain significant public health concerns for humans, there is a lack of comprehensive study in Indonesia. AIM: The aim of the study was to detectfrom rodents residing in the densely populated residential regions along the coastal areas of Banyuwangi Sub District. METHODS: A total of 169 rodents were captured across three villages of Kampung Mandar, Lateng and Kepatihan, using rat single live traps. After being euthanized and identified, the blood samples were collected from each rodent via cardiac puncture. Subsequently, the samples were subjected to native (direct blood microscopic examination), microscopic blood smear examination, and molecular analyses utilizing TRYP1S-TRYP1R (623 bp) and LEW1S-LEW1R (220 bp). RESULTS: The results demonstrated that two species of rodents were successfully captured:(65.68%) and(34.32%). Based on the native and microscopic blood smear examinations, the prevalence ofacross three villages was 23.08% and 24.26% for molecular analysis employing both primers, respectively. The highest prevalence was found in Kampung Mandar Village (31.18%), followed by Kepatihan (16.67%) and Lateng Villages (15.71%). CONCLUSION: Statistical analysis revealed thatwas more prevalent incompared to. In terms of sex, no statistically significant distinction was observed between female and male infected rodents of either species (> 0.05), indicating both species can serve as a source offor humans in the surveyed villages.