Peer-reviewed veterinary case report
Test to check dog immunity against canine parvovirus using latex
By Thomas, Jobin et al.·Published in Biologicals : journal of the International Association of Biological Standardization·2017·Indian Veterinary Research Institute, India·View original on PubMed →
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Original publication title: Determination of immune status in dogs against CPV-2 by recombinant protein based latex agglutination test.
- Species:
- dog
Plain-English summary
A group of puppies aged 6 to 20 weeks were tested for immunity against canine parvovirus-2 (CPV-2), a serious viral illness. Researchers developed a new, easier test using a special protein to check if the puppies had enough antibodies before vaccination. Out of 59 dogs tested, 85% of vaccinated dogs showed positive results for immunity. This new test was found to be quite accurate, making it a helpful tool for vets to determine if a puppy is ready for its CPV-2 vaccine.
People also search for: puppy parvovirus vaccination · how to check dog immunity · CPV-2 vaccine for puppies
Abstract
Canine parvoviral enteritis is a highly contagious viral illness caused by canine parvovirus-2 (CPV-2) which affects puppies of mainly 6-20 weeks of age. Vaccination is pivotal in preventing and controlling CPV-2 infection. Determination of antibody status is a critical determinant for successful vaccination. The hemagglutination inhibition (HI) test is 'gold standard' test for quantification of antibodies specific to CPV-2, although the execution of this test is not feasible under field conditions. The present study was undertaken to develop a point of care testing to determine immune status prior to CPV-2 vaccination or to detect seroconversion in immunized dogs by latex agglutination test (LAT) using recombinant antigen. Truncated portion of VP2 protein (tVP2) of CPV-2 was selected on the basis of antigenic indices, overexpressed the recombinant protein in E. coli system and was subsequently used in development of LAT. A total of 59 serum samples obtained from vaccinated (n = 54) and healthy unvaccinated (n = 5) dogs were tested. The positivity was observed in 85% (46/54) of these dogs with varying agglutination pattern. The overall sensitivity and specificity of latex agglutination test in comparison to HI test was recorded as 90% and 88% respectively with an agreement value of 90% (CI = 95%).
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/28689674/