Peer-reviewed veterinary case report
Development and application of a droplet digital polymerase chain reaction (ddPCR) for detection and investigation of African swine fever virus.
- Journal:
- Canadian journal of veterinary research = Revue canadienne de recherche veterinaire
- Year:
- 2018
- Authors:
- Wu, Xulong et al.
- Affiliation:
- College of Veterinary Medicine · China
Abstract
The aim of this study was to develop a droplet digital polymerase chain reaction (ddPCR) method to detect African swine fever virus (ASFV). The methods of ASFV real-time PCR and ddPCR were established and optimal reaction conditions were confirmed. Each method was evaluated for linearity, limit of detection, and specificity. The results indicated that ASFV ddPCR had a high degree of linearity (≥ 0.998) and specificity. The detection limit was 10 copies/reaction, which was approximately a 10-fold greater sensitivity than real-time PCR. This sensitive method could be used as an efficient molecular biology tool to diagnose ASFV, which is very important for preventing the spread of diseases across borders.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/29382972/