Peer-reviewed veterinary case report
Development and validation of a competitive ELISA kit for the serological diagnosis of ovine, caprine and bovine brucellosis.
- Journal:
- Journal of veterinary medicine. B, Infectious diseases and veterinary public health
- Year:
- 2006
- Authors:
- Portanti, O et al.
- Affiliation:
- Istituto Zooprofilattico Sperimentale dell'Abruzzo e · Italy
- Species:
- cat
Abstract
A competitive ELISA (Brucella-Ab c-ELISA) was standardized and validated for the detection of Brucella antibodies in cattle, sheep and goat sera using a monoclonal antibody (MAb 4B5A) produced against Brucella melitensis biotype 2. The specificity and sensitivity of the assay were 100% to a 67.5% cut-off point (B/Bo%). When compared with an indirect ELISA, the Brucella-Ab c-ELISA did not demonstrate cross-reactions when testing positive sera for antibodies to some Enterobacteriaceae. A comparison was made between the Brucella-Ab c-ELISA and the complement fixation and Rose Bengal tests. Results demonstrated that the Brucella-Ab c-ELISA is a valuable tool for the serological diagnosis of bovine and ovine/caprine brucellosis.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/17123429/