Peer-reviewed veterinary case report
Development and validation of an insulated isothermal PCR assay for the rapid detection of.
- Journal:
- Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
- Year:
- 2022
- Authors:
- Dao, Xiaofang et al.
- Affiliation:
- Department of Veterinary Medicine · China
Abstract
We developed a rapid insulated isothermal PCR (iiPCR) assay for on-site detection ofusing a primer and probe set targeting the superoxide dismutase (A) gene. Our iiPCR assay detectedclinical isolates successfully and produced negative results on other bovine or ovine respiratory pathogens, including,,,, andspp., indicating that the PCR reactions were specific. Additionally, our iiPCR assay detected as few as 21 copies of genomic DNA and 17.2 cfu/mL of bacterial culture, which was 10 and 100 times more sensitive than conventional PCR, respectively. Our iiPCR assay can be performed on a portable device in a total of 58 min and may be a useful tool for the detection ofin bovine and ovine respiratory disease in the field.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/35139720/