Peer-reviewed veterinary case report
Development of a novel LAMP diagnostic method for visible detection of swine Pasteurella multocida.
- Journal:
- Veterinary research communications
- Year:
- 2010
- Authors:
- Sun, Dongbo et al.
- Affiliation:
- College of Animal Science and Veterinary Medicine · China
Abstract
A set of four specific primers for six regions of kmt1 gene from a species specific region was designed for developing the loop-mediated isothermal amplification diagnostic method of swine Pasteurella multocida (Pm-LAMP). After the Pm-LAMP was carried out at 63°C for 1 h, the LAMP products could be visually confirmed using fluorescent dyes as detection reagent under UV-illumination. In sensitivity, the detection limit of the Pm-LAMP was 10 cfu/mL, and was 1 log less than that of the PCR method. In specificity, the Pm-LAMP did not amplify genomic DNA of swine common respiratory pathogens. Furthermore, based on results for clinical swab samples (n = 31) using PCR detection as golden standard, relative sensitivity of the Pm-LAMP was 100%, relative specificity of the Pm-LAMP was 90.9%, and percentage of observation agreement was 93.5% (Kappa = 0.85). The Pm-LAMP method should be a useful diagnostic tool for rapid and visible detection of swine Pasteurella multocida.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/20717843/