Peer-reviewed veterinary case report
New test detects canine parvovirus in dog poop compared to PCR
By Kumar, Manoj et al.·Published in Virologica Sinica·2010·Indian Veterinary Research Institute, India·View original on PubMed →
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Original publication title: Development of a polyclonal antibody-based AC-ELISA and its comparison with PCR for diagnosis of canine parvovirus infection.
- Species:
- dog
Plain-English summary
A new test called AC-ELISA was developed to detect canine parvovirus (CPV) in dog poop samples. This test uses special antibodies to identify the virus, and it was compared to another method called PCR. In a study with 152 samples, the AC-ELISA correctly identified 69 positive cases, while PCR found 78. The AC-ELISA was found to be a quick and reliable way to screen dogs for CPV infection, making it easier for vets to diagnose this serious illness.
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Abstract
A polyclonal antibody-based antigen-capture ELISA (AC-ELISA) has been developed for detection of Canine parvovirus (CPV) antigens in faecal samples of dogs. The assay uses rabbit anti-CPV polyclonal antibody as the capture antibody, guinea pig anti-CPV polyclonal antibody as tracing antibody and anti-guinea pig HRPO conjugate as the detection system. The optimum dilution of the capture antibody and the tracing antibody capable of detecting the CPV-2 antigens was found to be 1:1 600 and 1:400, respectively, in the check-board titration. In this study, a total of 152 samples (129 faecal samples and 23 cell culture supernatant) were tested both by AC-ELISA and by polymerase chain reaction (PCR). Of the samples tested, 69 and 78 samples were found positive by AC-ELISA and PCR, respectively. The AC-ELISA had relative sensitivity, relative specificity and accuracy of 88.4%, 100.0% and 91.4% respectively. The analytical sensitivity of AC-ELISA was estimated to be 10(2.8) TCID(50)/mL whereas PCR sensitivity was 10(0.8) TCID(50)/mL. The AC-ELISA is a simple, quick and reliable method for screening large numbers of faecal samples of dogs suspected of CPV infection.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/20960181/