Peer-reviewed veterinary case report
Test kit to detect canine coronavirus and parvovirus infections
By Li, Laiqing et al.·Published in Virology journal·2024·Belarusian State Technological University·View original on PubMed →
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Original publication title: Development of a time-resolved fluorescence immunoassay kit for detecting canine coronavirus and parvovirus through double labeling.
- Species:
- dog
Plain-English summary
A new test kit has been developed to detect two common viruses that cause stomach issues in dogs: canine coronavirus (CCV) and canine parvovirus (CPV-2). This kit can help veterinarians quickly identify which virus is making a dog sick, with high accuracy and sensitivity. It works by using special antibodies to capture the viruses and measure their levels in a sample. The test has shown to be reliable in clinical settings, making it a useful tool for diagnosing these infections.
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Abstract
OBJECTIVE: Canine enteric coronavirus (CCV) and canine parvovirus type 2 (CPV-2) are the main pathogens responsible for acute gastroenteritis in dogs, and both single and mixed infections are common. This study aimed to establish a double-labeling time-resolved fluorescence immunoassay (TRFIA) to test and distinguish CCV and CPV-2 diseases. METHODS: A sandwich double-labeling TRFIA method was established and optimized using europium(III) (Eu)/samarium(III) (Sm) chelates. CCV/CPV-2 antigens were first captured by the immobilized antibodies. Then, combined with Eu/Sm-labeled paired antibodies, the Eu/Smfluorescence values were detected after dissociation to calculate the CCV/CPV-2 ratios. The performance, clinical performance and methodology used for laboratory (sensitivity, specificity, accuracy and stability) testing were evaluated. RESULTS: A double-label TRFIA for CCV and CPV-2 detection was optimized and established. The sensitivity of this TRFIA kit was 0.51 ng/mL for CCV and 0.80 ng/mL for CPV-2, with high specificity for CCV and CPV-2. All the accuracy data were less than 10%, and the recovery ranged from 101.21 to 110.28%. The kits can be temporarily stored for 20 days at 4 °C and can be stored for 12 months at temperatures less than - 20 °C. Based on a methodology comparison of 137 clinically suspected patients, there was no statistically significant difference between the TRFIA kit and the PCR method. Additionally, for CCV detection, the clinical sensitivity was 95.74%, and the clinical specificity was 93.33%. For CPV-2 detection, the clinical sensitivity was 92.86%, and the clinical specificity was 96.97%. CONCLUSION: In this study, a double-label TRFIA kit was prepared for CCV and CPV-2 detection with high laboratory sensitivity, specificity, accuracy, stability, clinical sensitivity and specificity. This kit provides a new option for screening/distinguishing between CCV and CPV-2 and may help improve strategies to prevent and control animal infectious diseases in the future.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/38468354/