Peer-reviewed veterinary case report
New test detects Hepatozoon canis infection in dogs
By Singh, Manraj Deep et al.·Published in Ticks and tick-borne diseases·2019·Department of Veterinary Parasitology, India·View original on PubMed →
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Original publication title: Development of loop-mediated isothermal amplification (LAMP) assay for detection of Hepatozoon canis infection in dogs.
- Species:
- dog
Plain-English summary
A group of dogs was tested for a parasite called Hepatozoon canis, which can cause health issues. Researchers developed a new test called LAMP that was much better at detecting this parasite than traditional methods. In a study of 250 dogs, the LAMP test found the parasite in 75 dogs, while older tests only found it in 28 and 9 dogs, respectively. This new test could help veterinarians quickly and accurately diagnose Hepatozoon canis infections in dogs, leading to better treatment options.
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Abstract
The laboratory diagnosis of canine hepatozoonosis, caused by Hepatozoon canis is tedious, especially in chronic and latent infections. In the present investigation, a loop mediated isothermal amplification (LAMP) assay was developed and standardized targeting the partial 18S rRNA gene (GenBank accession no. KU096058). The LAMP primers specifically amplified H. canis DNA, whereas no amplification was detected in DNA samples from dogs infected with Babesia vogeli, B. gibsoni, Ehrlichia canis and Trypanosoma evansi, and no amplification was observed in DNA samples from H. canis-free dogs. The threshold sensitivity level of the assay was determined to be 15 fg of genomic DNA of H. canis. Furthermore, evaluation of blood samples collected from 250 dogs presented at Small Animal Clinics, Teaching Veterinary Clinical Complex, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab (India) was carried out for the presence of H. canis by microscopy, 18S PCR assay and LAMP assay. Of the total samples subjected to these tests, LAMP detected H. canis in 75 samples, while 18S PCR and microscopy detected H. canis in 28 and 9 samples, respectively. The present investigation has developed, for the first time, a highly sensitive, specific and rapid LAMP assay for the detection of H. canis, which has practical applications for the screening of field samples.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/30503892/