Peer-reviewed veterinary case report
Development of multiple ELISAs for the detection of antibodies against classical swine fever virus in pig sera.
- Journal:
- Virologica Sinica
- Year:
- 2012
- Authors:
- Yang, Zhen-hua et al.
- Affiliation:
- College of Life Sciences · China
Plain-English summary
Researchers developed new tests to check for antibodies against classical swine fever virus (CSFV) in pigs. They created specific proteins from the virus in a lab and used them to make several tests called ELISAs. These tests were shown to be very accurate by comparing them with other methods on samples from both vaccinated and unvaccinated pigs. The findings suggest that these ELISAs could be very useful for detecting CSFV infections and for monitoring the immune response in pigs that have been vaccinated. Overall, the tests worked well and could help keep pigs healthy.
Abstract
The major immunogenic proteins (E(rns), E2 and NS3) of classical swine fever virus (CSFV) (Shimen strain) were expressed in E. coli and purified by affinity chromatography. The recombinant antigens were applied to develop multiple enzyme-linked immunosorbent assays (ELISAs) for the detection of specific antibodies in pig sera. Optimum cut-off values were determined by receiver operating characteristic (ROC) analysis after testing 201 sera of vaccinated pigs and 64 negative sera of unvaccinated piglets. The multiple ELISAs were validated with 265 pig sera yielding high sensitivity and specificity in comparison with the virus neutralization results. The results demonstrated that multiple ELISAs can be a valuable tool for the detection of CSFV infection and serological surveys in CSFV-free countries or for the evaluation of the antibody responses in pigs induced by a live attenuated C-strain vaccination.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/22270806/