Peer-reviewed veterinary case report
How vets diagnose canine leishmaniasis using lymph node PCR and blood
By Maia, Carla et al.·Published in Veterinary journal (London, England : 1997)·2009·Unidade de Leishmanioses·View original on PubMed →
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Original publication title: Diagnosis of canine leishmaniasis: conventional and molecular techniques using different tissues.
- Species:
- dog
Plain-English summary
A dog with leishmaniasis, a disease caused by a parasite, was diagnosed using different tests including blood tests and tissue samples from the bone marrow and lymph nodes. The tests showed a high agreement in results, meaning they were reliable for confirming the disease. For pet owners, the lymph node PCR test is recommended for diagnosing leishmaniasis, while the bone marrow PCR test is a good option if the dog doesn't have swollen lymph nodes. These findings help veterinarians choose the best method for diagnosing and managing this condition in dogs.
People also search for: dog leishmaniasis symptoms · how to test for leishmaniasis in dogs · dog lymph node biopsy results
Abstract
Serology, bone marrow (BM)-, lymph node (LN)- and whole blood-polymerase chain reaction (PCR) were evaluated as potential reference tests for the diagnosis of canine leishmaniasis. A high degree of agreement (91.0%) was observed between Leishmania cultures and serology or BM/LN-PCR. In the light of these results as well as the access to biological test material and the cost of each method, LN-PCR is recommended for the diagnosis or therapeutic control of canine leishmaniasis, but BM-PCR is a suitable alternative in dogs without detectable adenomegaly. For large-scale epidemiological field studies, antibody detection is appropriate and whole blood-PCR can be used to complement the serological results.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/17936654/