Peer-reviewed veterinary case report
How PCR helps diagnose trypanosomosis in dogs
By P.V.Tresamol et al.·Published in Journal of Veterinary and Animal Sciences·2013·Dept. of Veterinary Epidemiology & Preventive Medicine College of Veterinary and Animal Sciences, Mannuthy-680651, Thrissur, Kerala, IN
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Original publication title: DIAGNOSIS OF CANINE TRYPANOSOMOSIS BY POLYMERASE CHAIN REACTION
- Species:
- dog
Plain-English summary
A German Shepherd dog was brought to the vet with symptoms like fever, cloudy eyes, pale gums, weight loss, and swollen lymph nodes. Tests on the dog's blood confirmed the presence of a parasite called Trypanosoma evansi, which causes a disease known as trypanosomosis. The vet used a specific test called polymerase chain reaction (PCR) to detect the parasite's DNA. The dog was treated successfully with a single dose of a medication called diminazine aceturate, and it recovered well.
People also search for: German Shepherd fever treatment · dog cloudy eyes causes · trypanosomosis in dogs treatment
Abstract
A clinical case of trypanosomosis in a German Shepherd dog and its diagnosis by polymerase chain reaction(PCR) was described.The dog was presented with clinical signs such as pyrexia, bilateral corneal opacity, pale mucous membranes, emaciation and lymphadenopathy. Wet film and stained smear examination of peripheral blood revealed Trypanosoma evansi. Blood sample was also subjected to PCR for detection of parasitic DNA using the forward and reverse Kin primers, Kin1: 5’-GCG TTC AAA GAT TGG GCA ATG-3’ and KIN-2: 5’-CGC CCG AAA GTT CAC C-3’, which bind to an internal transcribed spacer region (ITS1). Polymerase chain reaction showed positive result and the amplicon size for T.evansi was 540bp. The case was successfully treated with a single dose of diminazine aceturate @10 mg/kg BW
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