Peer-reviewed veterinary case report
New flow cytometry test to diagnose canine visceral leishmaniasis
By Moura, Henrique Bonifácio et al.·Published in Current pharmaceutical biotechnology·2024·Laborató, Brazil·View original on PubMed →
PetCaseFinder translated the abstract of this peer-reviewed paper into plain English so pet owners can read it. We do not publish original research — every detail traces back to the citation above. How we work →
Original publication title: Diagnosis of Canine Visceral Leishmaniasis by Flow Cytometry Serology using the rMELEISH Multiepitope Antigen Coupled in a Functional Bead.
- Species:
- dog
Plain-English summary
A study developed a new blood test to diagnose visceral leishmaniasis (VL) in dogs, which is a serious disease that can affect their health. The test showed a high success rate, correctly identifying 100% of dogs showing symptoms of the disease. It also detected 86% of dogs with mild symptoms and 80% of those without noticeable symptoms. This new test could help veterinarians more accurately diagnose VL in dogs, ensuring they receive the right treatment sooner.
People also search for: dog leishmaniasis symptoms · canine leishmaniasis diagnosis · blood test for dog diseases
Abstract
BACKGROUND: Visceral leishmaniasis (VL) is a zoonotic disease, with dogs being the main reservoir of the. OBJECTIVE: To develop a new flow cytometry test to diagnosis canine VL (CVL) diagnosis. METHODS: The current study addresses a new flow cytometry test using beads coupled to the multiepitope antigen rMELEISH. RESULTS: In the study set of samples a sensitivity (87.1%) and specificity (89.9%) was observed. Considering the dogs' clinical status, 20/20 (100.0%) of the symptomatic sera tested positive, while 19/22 (86.4%) of the oligosymptomatic and 16/20 (80.0%) of asymptomatic were positive. In the non-infected control, all samples (0/30) tested as negative. In the cross-reaction control, the test was more efficient in dogs infected with(2/10) and Trypanosoma cruzi (0/10), than those with(4/10) and(4/10). Dogs immunized with different vaccines (Leishmune, Leish-Tec, or LBSap) did not present serological reactivity. CONCLUSION: The flow cytometry serology through coupling the antigen rMELEISH in functional beads showed high accuracy in diagnosing CVL.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/38409720/