Effects ofEye Drop on Selenite-Induced Cataract Formation and Oxidative Stress-Related Parameters in Rabbits: An Experimental Study.

Abstract

PURPOSE: To evaluate the protective role ofeye drops on selenite-induced cataracts in suckling rabbits. METHODS: Fifteen male albino suckling rabbits with no signs of ocular inflammation were randomly assigned to three groups: controls (Group A), sodium-selenite group (Group B) and sodium-selenite plusgroup (Group C). By selenite treatment, cataract formation was experimentally induced and then graded. The grade of oxidative stress was defined in the lens, measuring the concentration of malondialdehyde, alpha-tocopherol, oxidized glutathione, ascorbic acid and hydrogen peroxide, and in blood samples as levels of alpha-tocopherol and malondialdehyde. RESULTS: Mean lens concentrations of GSSG, H2O2, and MDA levels in group B were significantly higher than in both group C and control. Ascorbic acid and alpha-tocopherol concentrations were lower in group B than in both group C and A. As plasma oxidative status markers, the level of MDA was higher in group B respected group C and A. The mean alpha-tocopherol levels in group B were significantly lower than in both group A and group C. CONCLUSIONS: In animals treated with-based eye drops, inflammation was inhibited, and lipid peroxidation was significantly reduced.leaves extract represents a good source of antioxidant components that may contrast sodium selenite-induced cataractogenesis in suckling rabbits.