Investigation of the Effects of N-acetylcysteine and Selenium on Vesicoureteral Reflux Nephropathy: An Experimental Study.

Abstract

INTRODUCTION: This study aimed to investigate the effects of N-acetylcysteine (NAC) and selenium (Se) on vesicoureteral reflux (VUR) nephropathy. METHODS: A total of 44 rabbits in 7 groups, namely group 1 (Control), group 2 (VUR&#xa0;+&#xa0;sterile urine), group 3 (VUR&#xa0;+&#xa0;sterile urine&#xa0;+&#xa0;NAC), group 4 (VUR&#xa0;+&#xa0;sterile urine&#xa0;+&#xa0;Se), group 5 (VUR&#xa0;+&#xa0;infected urine), group 6 (VUR&#xa0;+&#xa0;infected urine&#xa0;+&#xa0;NAC) and group 7 (VUR&#xa0;+&#xa0;infected urine&#xa0;+&#xa0;Se), were used.Tc Dimercaptosuccinic acid renal scan (DMSA), cystogram and urine culture were performed both at the beginning and end of the study. Left VUR was created surgically, and E.&#xa0;coli was inoculated in infected urine groups. NAC and Se were administered daily for 21 days. Malondialdehyde (MDA) measurement, inflammatory response scores (IRSs), and cicatrization response scores (CRSs) in renal tissues were evaluated. RESULTS: VUR did not reduce left renal uptake values in neither group 2 nor group 5. MDA levels of the left kidney were significantly higher in group 5 compared to group 1 (p&#xa0;=&#xa0;0.001). There was no significant difference in MDA levels between group 5 and group 6, and between group 5 and group 7. Left kidney IRSs were found to be higher in all other groups except group 2 compared to the control group (p&#xa0;<&#xa0;0.001). Left kidney CRSs were significantly higher in group 5 compared to group 2 (p&#xa0;=&#xa0;0.026), group 6 (p&#xa0;<&#xa0;0.001) and group 7 (p&#xa0;=&#xa0;0.006). CONCLUSION: A decrease in renal functions was not observed in VUR, even if there was infection. When CRSs were evaluated, NAC and Se had protective effects in terms of scar formation in VUR nephropathy. TYPE OF STUDY: Experimental animal study. LEVELS OF EVIDENCE: N/A.