Effects ofon characteristics of ovine mammary epithelial cells and milk production traits in dairy sheep.

Abstract

INTRODUCTION: Fibroblast growth factor 2 (FGF2), a pleiotropic growth factor, promotes the elongation of mammary ducts and therefore contributes to the morphological development of mammary. It plays a crucial role in the development of mammary gland and lactation processes. However, the impact ofon milk production traits in sheep remains unexplored. METHODS: In the study, the effects ofon the characteristics of ovine mammary epithelial cells (OMECs) were investigated. Meanwhile, penta-primer amplification refractory mutation system (PARMS) was used to look for single nucleotide polymorphisms (SNPs) in nine regions of ovine. Additionally, the associations of sequence variations inwith variations in average daily milk yield and seven milk composition traits were also investigated in 449 Yuansheng Aite dairy sheep. RESULTS: Among seven ovine tissues expressed by, it had the highest expression levels in liver and mammary gland tissue. Over-expressed of ovineincreased the viability, proliferation number and ratio of OMECs, as well as triglyceride content of OMECs. Conversely, silencing of ovineproduced the opposite effect to over-expression. A total of four novel SNPs were identified, with three located in intron 1 and one in intron 2. The genotypes and presence (or absence) of alleles fromwere found to affect milk production traits in sheep. Ewes with the genotypeat c.282 + 11,288 G/A had higher milk protein percentage, milk fat percentage, dry matter content and ash content. The presence of allele G at the c.282 + 11,288 G/A locus were associated with milk production traits described above in sheep. DISCUSSION: These findings indicate thatmay serve as a potential molecular marker for improving milk production traits in sheep.