Peer-reviewed veterinary case report
ELISPOT assays to enumerate bovine IFN-γ-secreting cells for the development of novel vaccines against bovine tuberculosis.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2012
- Authors:
- Vordermeier, Martin & Whelan, Adam O
- Affiliation:
- Department of Bacteriology · United Kingdom
Abstract
Enumeration of antigen-specific cells after vaccination is one of the prime immunological parameters determined when developing vaccines. Due to their exquisite sensitivity (limits of detection can be below 1/100,000 cells), ELISPOT assays are therefore an important tool in vaccine development programs. This is particularly the case for vaccines against diseases that require protective cell-mediated immunity, such as tuberculosis. This chapter describes ELISPOT assays detecting bovine IFN-γ.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/21956513/