Peer-reviewed veterinary case report
Rapid dog parvovirus test using body heat and no equipment
By Liu, Libing et al.·Published in Molecular and cellular probes·2018·Center of Inspection and Quarantine, China·View original on PubMed →
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Original publication title: Equipment-free recombinase polymerase amplification assay using body heat for visual and rapid point-of-need detection of canine parvovirus 2.
- Species:
- dog
Plain-English summary
A new test has been developed to quickly detect canine parvovirus (CPV-2), a serious virus that can make dogs very sick. This test uses body heat and can show results in just 15 minutes without needing any special equipment. In a study, the test was able to identify CPV-2 in 76.7% of fecal samples from dogs, which is better than some other methods. This easy-to-use test could be very helpful for vets, especially in places where resources are limited, allowing for faster diagnosis and treatment of sick dogs.
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Abstract
A visible and equipment-free recombinase polymerase amplification assay combined with a lateral flow strip (LFS RPA) was developed to detect canine parvovirus type 2 (CPV-2), which is the etiological agent of canine parvovirus disease. The CPV-2 LFS RPA assay was developed based on the VP2 gene and is performed in a closed fist using body heat for 15 min; the products are visible to the naked eye on the LFS within 5 min. The assay could detect CPV-2a, CPV-2b and CPV-2c, and there was no cross-reaction with the other viruses tested. Using the standard CPV-2 DNA as a template, the analytical sensitivity was 1.0 × 10copies per reaction, which was the same result as that of a real-time PCR. The assay performance was further evaluated by testing 60 canine fecal samples, and CPV-2 DNA was detected in 46 samples (76.7%, 46/60) by LFS RPA, which was the same result as that of the real-time PCR assay and higher than that of the SNAP method (48.3%, 29/60). The novel CPV-2 LFS RPA assay is an attractive and promising tool for rapid and convenient diagnosis of CPV disease, especially cage side and in underequipped laboratories.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/29705183/